Centrifuges should be placed on horizontally sturdy floors or platforms, and they should try to keep the machine in a horizontal position to avoid machine shake during centrifugation.
Turn on the power switch, install the required rotor as required, place the pre-balanced sample on the rotor sample holder (the centrifuge cartridge must be equilibrated with the sample) and close the lid.
Press the function selection key to set various requirements: temperature, speed, time, acceleration, and deceleration. The computer-controlled machine must also press the save key in order to remember the input information.
After the centrifuge is completely stopped, open the cover, remove the centrifugal sample, clean the rotor and the inner wall of the cavity with a soft clean cloth, and wait until the temperature in the cavity of the centrifuge is balanced with the room temperature and then cover the cover.
Note <br> <br> body should always be in a horizontal position, the voltage of the external power supply system to match, and requires a good grounding wire.
Before turning on the machine, check whether the rotating head is firmly installed and whether there is any foreign matter in the cavity.
Samples should be pre-balanced during centrifuge operation. Centrifuges must not be exposed to dangerous or volatile objects within 30 cm of the centrifuge. Centrifugal tubes with lids should be used to ensure that the fluid does not leak out to avoid corroding the cavity or causing accidents. .
Substances that are flammable, explosive and easily react with other substances cannot be centrifuged.
In the absence of appropriate protective measures, toxic, radioactive or pathogenic microorganisms cannot be centrifuged.
The lid is not opened artificially when the centrifuge is running.
If the rotor and cover have visible signs of corrosion or wear, stop using them immediately.
The glass or plastic centrifuge tube used should be able to withstand the appropriate centrifugal force and the appropriate size. Non-metallic rotors may not be irradiated with UV light.
If the density of the articles to be centrifuged is greater than the specification range of the specification, the centrifugal volume should be reduced according to the RCF calculation formula.
Ultracentrifuge process <br> <br> should pay attention in the medical field is mainly a centrifuge for separating cells and bacteria; purification and virus was harvested, RNA, DNA, plasmids and proteins and other biological macromolecules.
1. Composition of Centrifuge The centrifuge consists of a drive motor, a refrigeration system, a vacuum system (exceeding), a display system, an automatic protection system, and a control system. Necessary fittings are centrifugal rotors and centrifuge tubes. 1. Selection of Centrifugal Rotors The most commonly used rotors in centrifugation are angle heads and horizontal heads.
Angle heads are often used for differential centrifugation to separate samples with large differences in S (sedimentation coefficient) values ​​(1:10).
Horizontal heads are often used for density gradient or isodensity centrifugation.
Another type of vertical rotor is used less often. This rotor is mainly used for iso-centrifugal centrifugation to shorten the centrifugation time.
1. Selection of Centrifuge Tubes 2. Glass centrifuge tubes must not be used in high and ultra-fast centrifuges.
3. PA (polyamide) tube: chemically stable, translucent, resistant to high temperature sterilization.
4. PP (polypropylene) tube: chemically stable, translucent, resistant to high temperature sterilization.
5. PC (polycarbonate) tube: good transparency, hardness, high temperature sterilization. However, it is not resistant to strong acids and alkalis and some organic solvents. Mainly used for 50,000 (r/min) centrifugation.
6.CN (nitrocellulose) tube: The texture is soft and transparent, but it is not resistant to strong acids and alkalis and some organic solvents. It cannot be autoclaved. Suitable for sucrose, glycerol density gradient centrifugation. Should be transparent and conducive to collection.
Notes 1. Symmetry balance: When the centrifugal speed reaches 1-50000 (r/min), if the symmetry difference is 1g, the radius of the rotor is 5cm, then the centrifugal force formula F=m×RCF looks up: 10,000 /) RCF = 6000 into the formula F = 1 × 6000 = 6 (kg) 50,000 (r / min) RCF = 150000 into the formula F = 1 × 150000 = 150 (kg) at this time causes both sides of the imbalance up to 6- 150 (kg), this damage to the centrifuge is great, at least it will shorten the life of the centrifuge.
2. If the cover of the centrifuge tube is poorly sealed, the liquid cannot be filled (for high-speed centrifugation and use an angle head) to prevent spillage.
Loss of balance <br> <br> spillover consequences of pollution turned around and centrifugal chamber
Affect the normal operation of the sensor 1. When ultracentrifugation, the liquid must be filled with the centrifuge tube, the vacuum should be drawn when it is over, and only the top can avoid the deformation of the centrifuge tube.
2. Don't forget to turn the head cover when using the angle head. If it is not covered, there will be great eddy current resistance and frictional heating in the centrifugal cavity. This is equal to adding extra burden to the centrifuge motor and the refrigerator and affecting the centrifuge. Service life.
Turn on the power switch, install the required rotor as required, place the pre-balanced sample on the rotor sample holder (the centrifuge cartridge must be equilibrated with the sample) and close the lid.
Press the function selection key to set various requirements: temperature, speed, time, acceleration, and deceleration. The computer-controlled machine must also press the save key in order to remember the input information.
After the centrifuge is completely stopped, open the cover, remove the centrifugal sample, clean the rotor and the inner wall of the cavity with a soft clean cloth, and wait until the temperature in the cavity of the centrifuge is balanced with the room temperature and then cover the cover.
Note <br> <br> body should always be in a horizontal position, the voltage of the external power supply system to match, and requires a good grounding wire.
Before turning on the machine, check whether the rotating head is firmly installed and whether there is any foreign matter in the cavity.
Samples should be pre-balanced during centrifuge operation. Centrifuges must not be exposed to dangerous or volatile objects within 30 cm of the centrifuge. Centrifugal tubes with lids should be used to ensure that the fluid does not leak out to avoid corroding the cavity or causing accidents. .
Substances that are flammable, explosive and easily react with other substances cannot be centrifuged.
In the absence of appropriate protective measures, toxic, radioactive or pathogenic microorganisms cannot be centrifuged.
The lid is not opened artificially when the centrifuge is running.
If the rotor and cover have visible signs of corrosion or wear, stop using them immediately.
The glass or plastic centrifuge tube used should be able to withstand the appropriate centrifugal force and the appropriate size. Non-metallic rotors may not be irradiated with UV light.
If the density of the articles to be centrifuged is greater than the specification range of the specification, the centrifugal volume should be reduced according to the RCF calculation formula.
Ultracentrifuge process <br> <br> should pay attention in the medical field is mainly a centrifuge for separating cells and bacteria; purification and virus was harvested, RNA, DNA, plasmids and proteins and other biological macromolecules.
1. Composition of Centrifuge The centrifuge consists of a drive motor, a refrigeration system, a vacuum system (exceeding), a display system, an automatic protection system, and a control system. Necessary fittings are centrifugal rotors and centrifuge tubes. 1. Selection of Centrifugal Rotors The most commonly used rotors in centrifugation are angle heads and horizontal heads.
Angle heads are often used for differential centrifugation to separate samples with large differences in S (sedimentation coefficient) values ​​(1:10).
Horizontal heads are often used for density gradient or isodensity centrifugation.
Another type of vertical rotor is used less often. This rotor is mainly used for iso-centrifugal centrifugation to shorten the centrifugation time.
1. Selection of Centrifuge Tubes 2. Glass centrifuge tubes must not be used in high and ultra-fast centrifuges.
3. PA (polyamide) tube: chemically stable, translucent, resistant to high temperature sterilization.
4. PP (polypropylene) tube: chemically stable, translucent, resistant to high temperature sterilization.
5. PC (polycarbonate) tube: good transparency, hardness, high temperature sterilization. However, it is not resistant to strong acids and alkalis and some organic solvents. Mainly used for 50,000 (r/min) centrifugation.
6.CN (nitrocellulose) tube: The texture is soft and transparent, but it is not resistant to strong acids and alkalis and some organic solvents. It cannot be autoclaved. Suitable for sucrose, glycerol density gradient centrifugation. Should be transparent and conducive to collection.
Notes 1. Symmetry balance: When the centrifugal speed reaches 1-50000 (r/min), if the symmetry difference is 1g, the radius of the rotor is 5cm, then the centrifugal force formula F=m×RCF looks up: 10,000 /) RCF = 6000 into the formula F = 1 × 6000 = 6 (kg) 50,000 (r / min) RCF = 150000 into the formula F = 1 × 150000 = 150 (kg) at this time causes both sides of the imbalance up to 6- 150 (kg), this damage to the centrifuge is great, at least it will shorten the life of the centrifuge.
2. If the cover of the centrifuge tube is poorly sealed, the liquid cannot be filled (for high-speed centrifugation and use an angle head) to prevent spillage.
Loss of balance <br> <br> spillover consequences of pollution turned around and centrifugal chamber
Affect the normal operation of the sensor 1. When ultracentrifugation, the liquid must be filled with the centrifuge tube, the vacuum should be drawn when it is over, and only the top can avoid the deformation of the centrifuge tube.
2. Don't forget to turn the head cover when using the angle head. If it is not covered, there will be great eddy current resistance and frictional heating in the centrifugal cavity. This is equal to adding extra burden to the centrifuge motor and the refrigerator and affecting the centrifuge. Service life.
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