Analysis of Genetic Variation of Polymer Glutenin Subunits in Shanxi Wheat Varieties (Lines)

18(1): Journal of Sichuan Agricultural University June 27, 2000 Genetic variation of high molecular glutenin subunits in Shanxi wheat varieties (strain) Li Guangrong1, Yang Zujun S Chang Zhijian2 (1. Wheat Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, China 611830 2. Institute of Crop Genetics, Shanxi Academy of Agricultural Sciences, Taiyuan 04100, Shanxi, China (1), 13 strains are newly improved lines, and their high molecular glutenin subunit composition was analyzed, and according to Payne et al. The results showed that the composition of the high-molecular glutenin subunits in Shanxi's wheat finished product line was poor, mainly as follows: Null or 1 subunit at the Glu-A1 locus, 2x less; On B1, there are 7+8, 7+9, or 20 subunits, and there are fewer 5+10 subunits that are predominantly 2+12 subunits on Glu-D1, which may partially explain the baking of Shanxi wheat. Because of the poor quality, the wheat germplasm resources with high-quality glutenin subunits can be strengthened, and they can be introduced into wheat cultivars using comprehensive techniques such as SDS-PAGE to enrich the genetic basis of glutenin subunit composition in wheat cultivars. Into Further improve the level of quality of wheat breeding Shanxi. Further, five of which contain high-subunits, and stripe rust resistance varieties (lines) as Sichuan wheat breeding germplasm.

Wheat seed storage proteins include Gliadin and Glutenin, in which the genes controlling gliadin are located on the short arms of chromosomes 1 and 6 of homologous wheat, and the genes encoding glutenins are Located on the long arm of the corresponding chromosome. A large number of studies have shown that the content and composition of gliadin and glutenin determine the quality of wheat processing, while the glutenin genes closely related to function are mainly concentrated on the composition of high-molecular glutenin subunit genes. The sites of these high molecular glutenin subunits are only present on the long arms of chromosomes 1A, 1B, and 1D of wheat, and are named GluA1, GluB1, and GluD1, respectively. Although there are a large number of allelic variations at these sites, The positive correlation between the composition of some high-molecular glutenin subunits and bread baking quality has been widely confirmed. Many international germplasm resource centers have already carried out a large number of analyses of the composition of wheat high-molecular glutenin subunits. The selection method of high molecular glutenin subunits has been successfully applied to the quality improvement of British wheat varieties. Analysis of the composition of high-molecular glutenin subunits from high-quality bread wheat from Canada also revealed that the proportion of high-quality subunits was extremely high. The numbering of high-molecular glutenin subunits was performed with reference to the naming standard proposed by Payne et al. The quality score was calculated according to the calculation method of Payne 1 2 Results and analysis 2.1 The results of high molecular glutenin subunits in the distribution of individual subunits in the test materials are listed in Table 1, and the electrophoretograms of high molecular glutenin subunits of some materials are shown in Table 1. It can be seen that the band spectrum of the high-molecular glutenin subunits obtained by this electrophoresis method is fine, and the bands with similar mobility can be accurately distinguished. Therefore, all the subunits of the test materials can be clearly identified in the Sichuan Agricultural University's journal1. The allelic variation and frequency of wheat high molecular glutenin subunits were counted. The results are listed in Table 2 at the Glu-A1 site of high molecular glutenin subunits. The distribution of the popularized varieties and the finished product lines were consistent with that of the subunits. (null) Up to 50% of the subunit 1 Subunit 1 Average of 33% Subunit 2 Subunit combination ratios From the above, the subunit 7+8 is the highest, reaching 53.8% and the 7+ 9 account for a higher proportion , 26.9% and 30.8% of the popular varieties and finished product lines, respectively, while the subunit 20 accounted for 19.35% of the popular varieties, and the 19% of the middle subunits 20 and 6+8 of the finished product line accounted for a combination of Glu-D1. The variation is abundant, mainly including 5+10, 2+123+12 and 4+12. Among them, the combination of 2+12 subunits accounts for more than 50%. For the 5+10 subunit, 30.8% in the finished product line is higher than the 15.4% 4+12 band in the promoted variety, which is higher in the promoted variety than in the finished product line.

Table 1 Polymer Glutelin Subunits Analysis of Shanxi Wheat Varieties (Lines) Quality Rating Varieties (Lines) Quality Rating Jin 16 Jin 45 Jin 20 Jin 46 Jin 21 Jin 47 Jin 22 Jin 50 Jin 23 Jin 51 Jin 24 Jin 54 Jin 29 Yunjin 29 Yunjin 30 Yunjin 31 Yunjin 32 Yunjin 33 Yun 97 products 5 Jin 34 Yun Jin 36 Yun Jin 37 Yun Jin 38 Yun Jin 39 Yun Jin 40 Yun Jin 42 Yun Jin 43 2.2 Different subunit combinations in supply The distribution of the test materials among the popular varieties included 13 subunit combinations with an average rating of 6.6 points, and the finished product line with 6.8 points, slightly higher than the score of the promotion variety of 6.4 points. From the contribution of gluten sites, mainly due to the role of the Glu-D1 site, combined with the previous analysis, this is due to the frequency of 5+ large Shanxi wheat varieties (the frequency of the Taman subunits of the high molecular glutenin subunits of the line). Due to the increase.

Li Guangrong et al. Analysis of genetic variability of high molecular glutenin subunits in Shanxi wheat varieties (strains) Sichuan Agricultural University bookmark4 3 Discussion The comprehensive scoring of polymer glutenin subunits in Shanxi wheat was 66 and the overall level of Chinese wheat varieties was 6.8 Quite, there is a certain gap. The reason is that the gene locus of high molecular glutenin subunits is mainly expressed in subunits with lower quality scores, such as the subunit N of Glu-A1 and the subunit 20,6+8 on GluB1. The proportion of subunits 3+124+12 on 7+9 and GluD1 was higher, while the quality subunits with high quality scores, such as 2' of GluA1 and 5+10 of GluD1, accounted for a lower proportion. Therefore, from the improvement of the subunit composition of high-molecular glutenin, the proportion of high-quality subunits such as 25+10, etc. in wheat varieties can be further targeted and selected through targeted hybridization. At the same time, the introduction of high-quality subunits that are not found in Shanxi wheat varieties (lines), such as Pan Xinglai from Mexico or Canada, was analyzed by Smith. Chinese Journal of Genetics,199825(3):252258.Journal of Sichuan University(Natural Science Edition)199835(Collected Essays):3035 Li Guangrong et al. Genetic variation analysis of high molecular glutenin subunits in Shanxi wheat varieties(lines) Zhou Yonghong)